[SBYeast] Subtle Bioenergy on Yeast Growth and Viability
Project Overview
The goal of this project is to investigate whether bioenergy, also known as qi, prana, or life force energy, can influence the growth, viability, and metabolic health of living cells.
Yeast (Saccharomyces cerevisiae) will be used as a model organism due to its easy accessibility, well-established protocols, and relevance in both biology and biotechnology. By combining controlled meditation/bioenergy practices with quantitative biological assays, this study aims to better understand and quantify the effects of energetic practices.
Objectives
- Establish reliable protocols for assessing yeast health, including measurements of proliferation, viability, and metabolic activity.
- Determine whether exposure to focused bioenergy influences yeast growth or cellular activity compared to non-exposed controls.
- Investigate specific cellular responses, such as changes in ATP production and membrane potential, to identify potential underlying mechanisms.
- Contribute to the broader understanding of subtle bioenergy and its potential biological effects.
- Generate preliminary data to support future large-scale studies and potential scientific publications.
Project Phases
Phase 1 – Protocol Development and Initial Testing (Ongoing)
- Optimize meditation and bioenergy emission protocols;
- Set up sensors and measurement tools for environmental control and consistency;
- Conduct first pilot trials with yeast cultures;
- Assess: Proliferation (growth rate, cell count) and Viability (live vs. dead cells)
- Decision point: If promising effects are observed, proceed to Phase 2.
Phase 2 – Advanced Experimental Studies (pending evaluation/funding)
- Perform replicated experiments to validate Phase 1 results.
- Expand to more detailed assays, including:
- ATP production (cellular energy levels).
- Membrane potential changes using fluorescent dyes such as bis-(1,3-dibutylbarbituric acid) trimethine oxonol (DiBAC).
- Collect and analyze data to identify statistically significant trends.
Phase 3 – Data Analysis and Dissemination
- Comprehensive analysis of results and interpretation of findings.
- Preparation of a scientific manuscript or poster for publication and presentation.
- Identify potential follow-up research directions.
Planned Assays and Measurements
| Assay / Method | Purpose | Tools Required |
|---|---|---|
| Magnetic Field Disruption Test | Detect possible environmental energy effects | Sensors / EM field detector |
| Acidification Power Test | Measure metabolic activity via pH drop | pH meter |
| Microscopy with Methylene Blue + Glucose | Assess cell viability and vitality | Light microscope |
| ATP Quantification (Bioluminescence) | Measure intracellular energy levels | ATP assay kit + luminometer |
| Membrane Potential Assay (DiBAC dye) | Detect changes in cell membrane activity | Fluorescent dye + microscope |
Significance
This project explores a unique intersection between traditional bioenergetic practices and modern biology, providing a measurable way to test whether subtle energy fields have observable effects on living organisms. Even small, consistent changes in yeast physiology could open new research avenues in biophysics, consciousness studies, and integrative health sciences.
Next Steps
- Finalize Phase 1 protocols and collect baseline data.
- Evaluate preliminary results to refine methodology.
- Seek funding or collaboration for advanced Phase 2 assays.
- Build towards publication and broader scientific outreach.
About The Author
